rat embryonic aortic smooth muscle cell line a7r5 bcrc60082  (BioResource International Inc)

Journal: Oncotarget

Article Title: Estrogen modulates vascular smooth muscle cell function through downregulation of SIRT1

doi: 10.18632/oncotarget.22546

Figure Lengend Snippet: ( A ), or over a time course with 10 nM E2 (0, 12, 24, and 48 h) ( B ); we subjected the samples to western blot analysis to quantify SIRT1, GAPDH, and α-tubulin protein expression. ( C ) We analyzed the NAD + /NADH ratio in A7r5 cells subjected to the indicated treatments. Data are expressed as mean ± standard deviation (SD). Representative data shown are from experiments performed independently at least 3 times. * p < 0.05

Article Snippet: The rat embryonic aortic smooth muscle cell line A7r5 (BCRC60082) was purchased from Bioresource Collection and Research Center (Taiwan), and cultured in Dulbecco's modified Eagle's medium (Gibco Laboratories, Grand Island, NY, USA) with 10% fetal bovine serum (Gibco Laboratories) and antibiotics.

Techniques: Western Blot, Expressing, Standard Deviation

Journal: Oncotarget

Article Title: Estrogen modulates vascular smooth muscle cell function through downregulation of SIRT1

doi: 10.18632/oncotarget.22546

Figure Lengend Snippet: ( A ) Representative immunoblots of protein samples from A7r5 cells treated with 10 nM and 100 nM E2 that were stained with antibodies against caspase 3, Ki-67, cyclin D1, and GAPDH. ( B ) We quantified caspase 3, Ki-67, and cyclin D1 protein expression by computer-assisted densitometry analysis and presented their ratios to GAPDH, relative to the control group. ( C ) We performed flow cytometry on A7r5 cells treated with 10 nM and 100 nM E2, in order to assess the percentage in the G1 phase of the cell cycle.

Article Snippet: The rat embryonic aortic smooth muscle cell line A7r5 (BCRC60082) was purchased from Bioresource Collection and Research Center (Taiwan), and cultured in Dulbecco's modified Eagle's medium (Gibco Laboratories, Grand Island, NY, USA) with 10% fetal bovine serum (Gibco Laboratories) and antibiotics.

Techniques: Western Blot, Staining, Expressing, Control, Flow Cytometry

Journal: Oncotarget

Article Title: Estrogen modulates vascular smooth muscle cell function through downregulation of SIRT1

doi: 10.18632/oncotarget.22546

Figure Lengend Snippet: Wound healing assays were performed on A7r5 cells treated with 10 nM and 100 nM E2 for ( A ) 8 h and ( B ) 12 h. Black arrows indicate the wound edge. The residual gap between the migrating cells from the opposing wound edge is expressed as a percentage of the initial scraped area. Data are expressed as mean ± SD. Representative data shown are from experiments performed independently at least 3 times. * p < 0.05

Article Snippet: The rat embryonic aortic smooth muscle cell line A7r5 (BCRC60082) was purchased from Bioresource Collection and Research Center (Taiwan), and cultured in Dulbecco's modified Eagle's medium (Gibco Laboratories, Grand Island, NY, USA) with 10% fetal bovine serum (Gibco Laboratories) and antibiotics.

Techniques:

Journal: Oncotarget

Article Title: Estrogen modulates vascular smooth muscle cell function through downregulation of SIRT1

doi: 10.18632/oncotarget.22546

Figure Lengend Snippet: ( A ) We treated A7r5 cells with 10 nM E2 with and without resveratrol (Res, 15 μM); we then performed western blot analyses to quantify the levels of SIRT1 and GAPDH. ( B ) We analyzed the NAD + /NADH ratio in the presence and absence of resveratrol. Data are expressed as mean ± SD. Representative data shown are from experiments performed independently at least 3 times. * p < 0.05

Article Snippet: The rat embryonic aortic smooth muscle cell line A7r5 (BCRC60082) was purchased from Bioresource Collection and Research Center (Taiwan), and cultured in Dulbecco's modified Eagle's medium (Gibco Laboratories, Grand Island, NY, USA) with 10% fetal bovine serum (Gibco Laboratories) and antibiotics.

Techniques: Western Blot

Journal: Oncotarget

Article Title: Estrogen modulates vascular smooth muscle cell function through downregulation of SIRT1

doi: 10.18632/oncotarget.22546

Figure Lengend Snippet: ( A ) Representative immunoblots of protein samples from A7r5 cells exposed to 10 nM E2 with and without resveratrol (Res, 15 μM), labeled with antibodies against caspase 3, Ki-67, cyclin D1, and GAPDH. ( B ) We quantified caspase 3, Ki-67, and cyclin D1 protein expression by computer-assisted densitometry analysis and presented their ratios to GAPDH, relative to the control group. ( C ) Flow cytometric analysis of A7r5 cells in the G1 phase of the cell cycle after treatment with 10 nM E2 with and without resveratrol (Res, 15 μM).

Article Snippet: The rat embryonic aortic smooth muscle cell line A7r5 (BCRC60082) was purchased from Bioresource Collection and Research Center (Taiwan), and cultured in Dulbecco's modified Eagle's medium (Gibco Laboratories, Grand Island, NY, USA) with 10% fetal bovine serum (Gibco Laboratories) and antibiotics.

Techniques: Western Blot, Labeling, Expressing, Control

Journal: Oncotarget

Article Title: Estrogen modulates vascular smooth muscle cell function through downregulation of SIRT1

doi: 10.18632/oncotarget.22546

Figure Lengend Snippet: Wound healing assays were performed on A7r5 cells treated with 10 nM E2, with and without resveratrol (Res, 15 μM) after ( A ) 8 h and ( B ) 12 h. One representative experiment is shown. Black arrows indicate the wound edge. The residual gap between the migrating cells from the opposing wound edge is expressed as a percentage of the initial scraped area. Data are expressed as mean ± SD. Representative data shown are from experiments performed independently at least 3 times. * p < 0.05

Article Snippet: The rat embryonic aortic smooth muscle cell line A7r5 (BCRC60082) was purchased from Bioresource Collection and Research Center (Taiwan), and cultured in Dulbecco's modified Eagle's medium (Gibco Laboratories, Grand Island, NY, USA) with 10% fetal bovine serum (Gibco Laboratories) and antibiotics.

Techniques:

Journal: Oncotarget

Article Title: Estrogen modulates vascular smooth muscle cell function through downregulation of SIRT1

doi: 10.18632/oncotarget.22546

Figure Lengend Snippet: Representative immunoblots of A7r5 cells treated with 10 nM E2, with and without resveratrol (Res, 15 μM), labeled with antibodies against SIRT1, p-Akt, Akt, p-ERK, ERK, and GAPDH.

Article Snippet: The rat embryonic aortic smooth muscle cell line A7r5 (BCRC60082) was purchased from Bioresource Collection and Research Center (Taiwan), and cultured in Dulbecco's modified Eagle's medium (Gibco Laboratories, Grand Island, NY, USA) with 10% fetal bovine serum (Gibco Laboratories) and antibiotics.

Techniques: Western Blot, Labeling